Biotechnology Reagents (IGFs) >> Product Support >> Application Notes >> #2001 Human IGF-I antibody for Immunohistochemistry (IHC)

IGF-I antibody for immunohistochemistry has been characterized for the detection of IGF-I in
1) TissueTek OCT sections of human and rat skin by Extravidin-Cy3 staining
2) paraffin sections of rat tissues by immunoperoxidase staining and
3) paraffin sections of barramundi (fish) tissues by immunoperoxidase staining


The antibody was purified from a polyclonal antiserum obtained from rabbits immunized with a recombinant immunogenic analog of human IGF-I conjugated to ovalbumin. Each vial of lyophilized antibody contains 200 µg IgG and is stable in the lyophilized state for at least 2 years* at 2-4°C.

*Longer term stability is expected to be demonstrated.

1. Reconstitution:

Dissolve the vial contents in 200 µl 1% (w/v) BSA / PBS (10 mM phosphate buffer pH 7.4, 150 mM NaCl. Make up dilutions of the antibody as required in 1% BSA / PBS and mix thoroughly.

2. Recommended titre for use in Immunohistochemistry (IHC):

For immunohistochemistry we recommend the antiserum be used at a final dilution of 1/50 - 1/200.

3. Protocols

See Protocols:
#3050 Immunohistochemical (IHC) Detection of human or rat IGF-I in OCT sections by Extravidin-Cy3 staining.

#3051 Immunohistochemical (IHC) Detection of IGF-I in paraffin embedded sections of rat tissues by immunoperoxidase staining.

#4110 Immunohistochemical Detection of IGF-I in paraffin embedded Fish (Lates calcarifer) kidney sections by immunoperoxidase staining.

* NOT FOR USE IN HUMANS


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