Biotechnology Reagents (IGFs)
>>
Product Support
>>
Application Notes
>>
#1100: Handling of Novozymes GroPep IGF Binding Proteins 1-6
General Precautions:
* Avoid freeze-thaw cycles
Stability / Storage: In lyophilized form, the IGFBP is stable for at least 2 years at 2-4°C, or 6 months at room temperature.
Handling Procedure: Novozymes GroPep IGF Binding Proteins are supplied as lyophilized preparations in glass or siliconized plastic vials. Care should be taken when opening the vial and reconstituting the IGFBP to avoid any losses.
Reconstitution and Stock Solutions: Reconstitute the lyophilized IGFBP in sterile-filtered 10 mM HCl at a concentration of 0.1 - 1 mg/ml (see below). This working solution is stable at 4°C for at least one week. For longer term storage it is recommended the solution is snap-frozen and stored at -80°C. Avoid additional freeze-thaw cycles. Stock solutions of peptide can be stored for at least six months at -80°C.*
IMPORTANT: Irrespective of size, always reconstitute the entire vial in situ and then aliquot. Each vial contains the stated amount of protein indicated on the label and up to 20 % more by mass due to the residual moisture and counter ions retained during the freeze drying process. Therefore DO NOT estimate protein content by weight or gravimetric means as this will underestimate the actual protein content by up to 20 %.
Handling of 20 µg pack sizes: While a 1 mg/ml stock solution is always preferable, it is often not practicable when reconstituting smaller quantities of peptide. For 20 µg pack sizes the peptide should be dissolved in 100 µl of 10 mM HCl and treated as above.
Sterilization: Novozymes GroPep products are prepared using aseptic techniques and should therefore be adequate for most purposes.
*Longer term stability of lyophilized and frozen stock solutions is expected on further testing.
* NOT FOR USE IN HUMANS
© Novozymes GroPep Limited
28 Dalgleish Street
Thebarton SA 5031
PO Box 10065 BC
Adelaide SA AUSTRALIA
Telephone +61 (0)8 8354 7700
Facsimile +61 (0)8 8354 7788
Email:
AUAD-bioreagents@novozymes.com
Website: www.gropep.com.au
|
