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Description:

Human Insulin-like Growth Factor-I (IGF-I) is a single chain, 70 amino acid polypeptide that stimulates growth and differentiation in many cell types. Human IGF-I shares 76 % sequence homology with human Insulin-like Growth Factor-II (IGF-II) and approximately 50 % with human proinsulin. IGF-I is considered to be an autocrine/paracrine and endocrine regulator of growth and protein metabolism acting through the Type 1 IGF receptor.

References:

Humbel R. E. (1990) Eur. J. Biochem., 190, 445 – 462
Stewart C. E. H. & Rotwein P. (1996) Physiol. Rev., 76, 1005 – 1026

Source:

Produced recombinantly in E. coli

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7649 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay – ED₅₀ < 10 ng/ml
IGF binding protein assay – ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 30 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Detection:

#3050: Immunohistochemical (IHC) Detection of human or rat IGF-I in OCT sections by Extravidin - Cy3 staining

#3005: Procedure for Western Immunoblotting IGF-I

#3004: Procedure for Western Ligand Blotting using biotinylated IGF-I or IGF-II

#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

#3001: Procedure for the Iodination of IGFs

Related Products:

Human IGF-I (Media Grade)

Biotinyl human IGF-I

Any combination of 3 different IGF-Is and/or IGF-I analogs (all Receptor Grade)

IGF-I, antibody for Immunohistochemistry

IGF-I, anti-human, monoclonal

IGF-I, anti-human

IGF-I Immunohistochemistry Pack

Human IGF-I pre-RIA Pack

Human IGF-I & IGF-II Pack

Human IGF-I Pack

Product Performance:
      IGF-I Binding to IGFBP-2:




      IGF Specificity Curves:




      Protein Synthesis:




      Radioreceptor Assay:




      Protein Synthesis:




      Protein Synthesis:




      Protein Synthesis:




      Radioreceptor Assay:




      Protein Synthesis:




      Radioreceptor Assay:




      Binding of IGF-I to IGFBP-2:




      Protein Synthesis:




      Radioreceptor Assay: