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Description:

GroPep scientists and engineers have produced Animal/Media Grade Human IGF-I to provide a high quality reagent for use in studies on cellular and animal growth and for inclusion in cell culture medium.

References:

Humbel R.E. (1990) Eur. J. Biochem., 190, 445-462
Stewart C.E.H. & Rotwein P. (1996) Physiol. Rev., 76, 1005-1026

Source:

Produced recombinantly

Purity:

> 85 % (by HPLC)

Molecular Weight:

7649 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay – ED₅₀ < 10 ng/ml
IGF binding protein assay – ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 30 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Related Products:

Human IGF-I(Receptor Grade)

Product Performance:
      Protein Synthesis:




      Radioreceptor Assay: