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Description:

Chicken Insulin-like Growth Factor-II (Chicken IGF-II) is a polypeptide of 66 amino acids, twelve of which differ from the amino acid sequence of human IGF-II. Chicken IGF-II stimulates growth and differentiation in many cell types, including rat L6 myoblasts and chicken embryo fibroblasts.

References:

Upton Z. et al. (1995) J. Mol. Endocrinol. 14, 79-90

Source:

Produced recombinantly in E.coli.

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7512 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ < 20 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 150 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum. (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Detection:

By Radioimmunoassay (RIA)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Detection:

#3001: Procedure for the Iodination of IGFs

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II

Related Products:

IGF-II, anti-human

Chicken IGF-II pre-RIA Pack

Chicken IGF-I & IGF-II Pack

Chicken IGF-II Pack

Chicken IGF-I

Product Performance:
      Binding of IGF-II to Ovine Placental Membranes:




      Chicken IGF-I binding of 125I-Labelled IGF-I: