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Description:

Human Insulin-like Growth Factor-II (IGF-II) is a single chain 67 amino acid polypeptide that shares structural homology with insulin and human IGF-I. Human IGF-II is widely distributed in many tissues throughout the body and acts on numerous cell types through the Type 1 IGF-I receptor. IGF-II also binds specifically to the IGF-II / cation-independent mannose-6-phosphate receptor as well as the family of six IGF binding proteins, but the physiological function of IGF-II as a result of all these interactions has not been clearly established.

References:

Humbel R.E. (1990) Eur. J. Biochem. 190, 445-462
Stewart C.E.H. & Rotwein P. (1996) Physiol. Rev. 76, 1005-1026

Source:

Produced recombinantly in E.coli.

Purity:

>95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

7469 daltons – homogeneity confirmed by Mass Spectrometry

N-terminal sequence analysis:

7 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ < 20 ng/ml
Type 2 IGF receptor binding assay: ED₅₀ < 70 ng/ml
IGF binding protein assay: ED₅₀ < 10 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 150 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum.

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Detection:

#3001: Procedure for the Iodination of IGFs

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

#3003: Procedure for Western Ligand Blotting using iodinated IGF-I or IGF-II

#3004: Procedure for Western Ligand Blotting using biotinylated IGF-I or IGF-II

Related Products:

Di-biotinyl human IGF-II (Receptor Grade)

Mono-biotinyl human IGF-II

Human IGF-II (Media Grade)

IGF-II, anti-human

Human IGF-II pre-RIA Pack

Human IGF-I & IGF-II Pack

Human IGF-II Pack

Product Performance:
      Radioreceptor Assay:




      Protein Synthesis:




      IGF Specificity Curves:




      Protein Synthesis:




      Radioreceptor Assay: