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Description:

Human Des(1-6) Insulin-like Growth Factor-II (Des(1-6)IGF-II) is a 61 amino acid analog of human IGF-II, lacking the first six N-terminal amino acids. This analog of IGF-II binds to the Type 1 and Type 2 IGF receptors with similar affinity to IGF-II. Human Des(1-6)IGF-II exhibits reduced affinity for IGF binding proteins (IGFBPs), which modulate the actions of IGFs.

References:

Francis G. L. et al. (1993) Biochem. J. 293, 713-719

Source:

Produced recombinantly in E.coli.

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

6765 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Type 1 IGF receptor binding assay: ED₅₀ < 50 ng/ml
Type 2 IGF receptor binding assay: ED₅₀ < 100 ng/ml
IGF binding protein assay: ED₅₀ > 1000 ng/ml
Stimulation of protein synthesis in rat L6 myoblasts: ED₅₀ < 75 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder.
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum.

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs

Detection:

#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)

Product Performance:
      Protein Synthesis: