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Antibodies
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IGF Family
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IGF-I
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References:
Degger, B. et al. (2000) Gen. Comp. Endocrinol. 117, 395-403
Source:
Antiserum (Rabbit)
Immunogen:
Recombinant barramundi (Lates calcarifer) IGF-I
State and Appearance:
Lyophilized powder
Specificity:
As determined by RIA:
The antiserum is equipotent with [125I]-labelled barramundi, bream (Pagrus auratus), salmon/trout (Oncorhynchus & Salmo sp.), tilapia (Oreochromis mossambicus) and tuna (Thunnus maccoyii) IGF-I tracer (80 Ci/g).
< 1% cross-reactivity with human IGF-I and human IGF-II
No cross-reactivity with salmon insulin
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
After reconstitution, aliquot and store at -20°C
Avoid freeze-thaw cycles
Reconstitution:
Dissolve the vial contents in 500 µl RIA buffer (30 mM sodium phosphate pH 7.5 containing 10 mM EDTA, 0.2 g/l protamine sulphate, 0.5 ml Tween 20, 0.25 g/l sodium azide) to give an antiserum stock of 1/50.
Application and Titre:
Radioimmunoassay 1/10,000
At a dilution of 1/10,000 the antiserum binds 20 % of fresh [125I]-labelled barramundi IGF-I tracer (80 Ci/g).
Half-maximal inhibition (ED50) of radioligand binding is produced by 1.7 ng/ml of barramundi IGF-I.
Protocol:
#3002: Determination of IGF-I or IGF-II in a Range of Species by Radioimmunoassay (RIA)
Related Products:
Barramundi IGF-I
Barramundi IGF-I Pack
Product Performance:
Cross-Reactivity:
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