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Description:

Recombinant methionyl black bream (Acanthopagrus butcheri) Growth Hormone (m-brGH) is a 21.41 kDa protein that stimulates growth in fish. This black bream GH shares 97% amino acid sequence identity with gilthead sea bream (Sparus aurata), 94% with red sea bream (Pagrus major), 93% with the tuna (Thunnus thynnus), 91% with barramundi (Lates calcarifer), 63% with the salmon (Oncorhynchus keta) and 32% with human GH.

References:

Knibb W. et al. (1991) J DNA Sequencing and Mapping, 2, 121-123

Source:

Produced recombinantly in E.coli

Purity:

95% monomer (by Gel Filtration Chromatography)

Molecular Weight:

21.41 kDa confirmed by Mass Spectrometry

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder under dry nitrogen at a slight vacuum
(-25kPa) from 5 mM sodium phosphate buffer pH 8.5, containing m-brGH/ mannitol/ glycine (1:5:1 w/w)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1400: Handling of Novozymes GroPep Growth Hormones

Protocols:

#3400: Procedure for Iodination of Growth Hormones

#3403 Procedure for Radioimmunoassay (RIA) of Bream (Acanthopagrus butcheri) Growth Hormone

Related Products:

Growth Hormone, anti-bream

Bream Growth Hormone pre-RIA Pack

Bream Growth Hormone Pack