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Description:
Native human Insulin-like Growth Factor Binding Protein-1 (IGFBP-1) is a 218 amino acid protein that is a member of a family of six circulating proteins which bind IGF-I and IGF-II with high affinity and modulate their metabolic and mitogenic effects. The liver, ovarian granulosa cells, decidualized endometrium and a number of other cell types produce native human IGFBP-1. IGFBP-1 binds both IGF-I and IGF-II. Serum IGFBP-1 levels are regulated by insulin-induced inhibition of IGFBP-1 production. IGFBP-1 appears to inhibit smooth muscle migration in response to IGF-I or IGF-II and to stimulate wound healing in response to IGF-II.
References:
Liu S. et al. (1991) Biochem. Biophys. Res. Comm. 174, 673-679
Schuller A. G.et al. (1993) Growth Regulation 3, 32-34
Source:
Purified from human amniotic fluid of individuals that have been shown by certified tests to be negative for hepatitis.
Purity:
> 95% (by FPLC and SDS-PAGE)
Consists of 3 - 4 phosphorylated forms.
Molecular Weight:
25.3 kDa
Biological Activity:
Inhibition of IGF-I stimulated proliferation of NUC-1 cells: IC50 ~ 100 ng/ml
Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures: IC50 (1% serum) ~ 300 ng/ml.
State and Appearance:
Lyophilized white powder dried from 10 mM HCl and stored under dry nitrogen at a slight vacuum (-25 kPa).
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized).
Reconstitution:
#1100: Handling of GroPep Bioreagents IGF Binding Proteins 1-6
Detection:
#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining
#3110: Protocol for Western Immunoblotting using human IGFBP antisera
Related Products:
IGFBP-1, anti-human
IGFBP-1, antibody for Immunohistochemistry
Human IGFBP-1 Pack
IGFBP-1 Immunohistochemistry Pack
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