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Description:

Bovine Insulin-like Growth Factor Binding Protein-2 (bIGFBP-2) is a 284 amino acid protein that is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. bIGFBP-2 binds both human and bovine IGF-I and IGF-II and modifies their biological activity and bioavailability.

Recombinant bovine IGFBP-2 is available in both Animal/Media Grade and Receptor Grade.

The Animal/Media Grade product contains mature bovine IGFBP-2 of correct Molecular Weight as the major species. As a result of alternative processing by the CHO cells used for the bIGFBP-2 production, three other minor bIGFBP-2 isoforms are also present:

1) One with a three amino acid C-terminal deletion, denoted Des(1-3) bIGFBP-2.
2) One with a single amino acid N-terminal extension, denoted A-bIGFBP-2.
3) One with a four amino acid N-terminal extension, denoted GARA-bIGFBP-2.

References:

Upton Z. et al. (1990) J. Mol. Endo. 5, 77-84
Clemmons D. R. et al. (1992) Endocrinology, 131, 890-895
Bourner M. J. et al. (1992) J. Cell. Biochem. 48, 215-226

Source:

Produced recombinantly in mammalian cells.
Purified by affinity and other chromatographic steps.

Purity:

>95% by HPLC and Mass Spectrometry

Molecular Weight:

30.776 kDa - Confirmed by Mass Spectrometry

Biological Activity:

IGF-I Binding Assay : ED₅₀ < 10 nM

State and Appearance:

Lyophilized white powder dried from 0.1% TFA / 30% acetonitrile and stored under dry nitrogen at a slight vacuum (-25 kPa).

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).

Reconstitution:

#1100: Handling of GroPep Bioreagents IGF Binding Proteins 1-6

Related Products:

Bovine GARA-IGFBP-2 (Receptor Grade)

Bovine IGFBP-2 (Media Grade)