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Description:

Human Insulin-like Growth Factor Binding Protein-3
(hIGFBP-3) is a 264 amino acid protein that is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. Human IGFBP-3 is a 40-44 kDa glycoprotein that is the most abundant IGFBP in serum and milk. It is produced by non-parenchymal hepatic cells and circulates in serum, binding IGF-I or IGF-II in conjunction with an acid labile subunit (ALS) to form a (1:1:1) 150 kDa circulating complex at a serum concentration of about 100 nM. Free IGF-I has a half-life of ~ 10 min in serum. This can be increased to ~ 25 min if bound to IGFBP-3 and to ~ 15 h if complexed with IGFBP-3 and ALS.

References:

Rechler M.M. & Clemmons, D.R. (1998) TEM 9,
176-183
Wood W.I. et al. (1998) Mol. Endocrinol. 2, 1176-1185

Source:

Produced recombinantly in mammalian cells.
Purified by affinity chromatography and HPLC.

Purity:

> 95% (by HPLC and N-terminal sequence).

Molecular Weight:

43 - 45 kDa

Biological Activity:

Characteristic doublet of glycoforms detected by Western ligand blot using mono-biotinyl IGF-II as probe.

State and Appearance:

Lyophilized white powder dried from 0.1M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa).

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1100: Handling of GroPep Bioreagents IGF Binding Proteins 1-6

Detection:

#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining

#3101: Immunohistochemical (IHC) Detection Of IGFBP-3 In Human Liver (OCT) Sections Using Extravidin - Cy3 Staining

#3110: Protocol for Western Immunoblotting using human IGFBP antisera

Related Products:

IGFBP-3, anti-human, monoclonal

IGFBP-3, antibody for Immunohistochemistry

IGFBP-3, anti-human

Human IGFBP-3 Pack

IGFBP-3 Immunohistochemistry Pack