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Description:

Rat Insulin-like Growth Factor Binding Protein-5 (rIGFBP-5) is a 252 amino acid protein that is one of the six “classical” IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. Rat IGFBP-5 preferentially binds IGF-II and it also binds with high affinity to extracellular matrix components which protect it from proteolysis. Like human IGFBP-5, rat IGFBP-5 has a nuclear targeting sequence.

References:

Kiefer M. et al. (1991) Biochem. Biophys. Res. Commun. 176, 219-225
Song, H. et al. (2000) J. Mol Endocrinol. 24, 43-51

Source:

Produced recombinantly in E.coli.
Purified by affinity and other chromatographic steps.

Purity:

> 90% (by SDS PAGE)

Molecular Weight:

29 kDa (non-glycosylated; five amino acid N-terminal extension)

Biological Activity:

Binds ¹²⁵I-IGF-I in western ligand blot. Binding affinity for IGF-I - 0.2nM (Biosensor).

State and Appearance:

Lyophilized white powder dried from phosphate buffered saline and stored under dry nitrogen at a slight vacuum (-25 kPa)

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1100: Handling of GroPep Bioreagents IGF Binding Proteins 1-6

Detection:

#3100: Immunohistochemical (IHC) Detection of IGFBPs In Paraffin Sections Of Rat Embryonic Tissues Using Extravidin-Cy3 Staining

Related Products:

IGFBP-5, antibody for Immunohistochemistry

Rat IGFBP-5 Immunohistochemistry Pack