Upton Z. et al. (1996) J. Endocrinol. 149, 379-397
Source:
Rabbit antiserum
Immunogen:
Recombinant human IGF-II
Specificity:
As determined by RIA:
100 % human IGF-II, rat IGF-II, chicken IGF-II and porcine IGF-II
85 % bovine IGF-II
< 0.1 % ovine insulin, human IGF-I, chicken IGF-I, rat IGF-I
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C
Avoid freeze-thaw cycles
Reconstitution:
Dissolve in a suitable buffer appropriate for the desired application. See:
Application and Titre:
Radioimmunoassay 1/7,500 At a dilution of 1/80,000 the antiserum binds 20 % of fresh [125 I]hIGF-II tracer (80 Ci/g). Half-maximal inhibition (ED50) of radioligand binding is produced by ~ 4 ng/ml of hIGF-II.
