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Antibody Type and Class:

Mouse monoclonal antibody (IgG1)

Immunogen:

Human IGFBP-3 plus epitope of human IGFBP-3

State and Appearance:

Lyophilized powder

Specificity:

As determined by EIA:
<0.05% Cross-reactivity with hIGFBPs 1,2,4,5 or 6

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized).
After reconstitution, aliquot and store at -20°C or -80°C.
Avoid freeze-thaw cycles

Reconstitution:

Reconstitute the vial contents in 200 µl sterile water. Allow to stand at room temperature for at least 10 minutes before use. Dilute in a buffer solution appropriate for the desired application.

Application and Titre:

Characterized for EIA (1/8,000) and immunoblotting (1/5,000).
Detects 60 ng human IGFBP-3 in western immunoblot (at a final dilution of 1/5,000).

Protocol:

#3120 Procedure for Enzyme Immunoassay (EIA) of IGFBPs

Related Products:

Human IGFBP-3

IGFBP-3 Immunohistochemistry Pack

IGFBP-3, antibody for Immunohistochemistry

Human IGFBP-3 Pack

IGFBP-3, anti-human