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References:

Upton Z. et al. (1996) J. Endocrinol. 149, 379-397

Source:

Antiserum (Rabbit), Affinity Purified

Immunogen:

An immunogenic form of recombinant human IGF-I

State and Appearance:

Lyophilized powder

Specificity:

As determined by immunohistochemistry:
Antibody cross-reacts with human, mouse, rat and barramundi (fish) IGF-I
< 0.05 % with human IGF-II

Storage/Stability:

At least 6 months at 2 - 4°C (lyophilized)
After reconstitution, aliquot and store at -20°C
Avoid freeze-thaw cycles

Reconstitution:

Reconstitute the 200 µg vial in 200 µl sterile distilled water.
Dilute in a suitable buffer for immunohistochemistry studies.

Application and Titre:

Recognises IGF-I in frozen and paraffin embedded human, mouse, rat and barramundi (fish) tissue
(Images below)
Immunohistochemistry 1/50 – 1/200
Use LONG®R³IGF-I as competitive control

Protocols:

#3050: Immunohistochemical (IHC) Detection of human or rat IGF-I in OCT sections by Extravidin - Cy3 staining

#3051: Immunohistochemical (IHC) Detection of IGF-I in paraffin embedded sections of rat tissues by Immunoperoxidase staining

#3102: Immunohistochemical (IHC) detection of human or rat IGFBPs and IGF-I, and rat IGF-II in formalin fixed, paraffin embedded human and rat tissue sections by horseradish peroxidase staining

#4110: Immunohistochemical (IHC) Detection of IGF-I in paraffin sections of Barramundi (fish) kidney by Immunoperoxidase staining

Related Products:

Human IGF-I

Rat IGF-I

Barramundi IGF-I

Human LONG®R³IGF-I

IGF-I Immunohistochemistry Pack

Immunohistochemistry Images:

Promotional flyer: IGF-I, antibody for Immunohistochemistry

Product Performance:
      IGF-I in human skin (Wound repair):




      IGF-I in human kidney:




      IGF-I in rat skin:




      IGF-I in rat Sebaceous gland:




      IGF-I in mouse kidney:




      IGF-I in barramundi (fish) kidney: