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Description:
Salmon / Trout (Oncorhynchus sp. & Salmo sp.) Insulin-like Growth Factor-II (Salmon / Trout IGF-II) is a 70 amino acid polypeptide. Salmon / Trout IGF-II can be used for a variety of in vivo and in vitro applications.
References:
Wilkinson R. et al. (2004) Protein Expression and Purification, 35, 334-343
Source:
Produced recombinantly in E.coli
Purity:
> 95 % (by HPLC and N-terminal sequence analysis)
Molecular Weight:
7866 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis:
5 residues > 95 % single sequence
Biological Activity:
Stimulation of protein synthesis in rat L6 myoblasts: ED50 < 256.6 ng/ml
State and Appearance:
Lyophilized white powder
Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
Reconstitution:
#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs
Detection:
#3020 General Procedure for Microplate Enzyme Immunoassay (EIA)
#4120 Procedure for Enzyme Immunoassay (EIA) of Salmon/Trout IGF-II
Related Products:
IGF-II, Anti-Salmon/Trout
Salmon / Trout IGF-II Pack
Product Performance:
Protein Synthesis:

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