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References:

Wilkinson R. et al. (2004) Comp. Biochem. Physiol. B Biochem. Mol. Biol., 139, 193 - 201.

Source:

Rabbit antiserum, Purified IgG (100 µl)
Total IgG: Approximately 0.52 mg per vial

Antibody Type and Class:

IgG

Immunogen:

Peptide epitope from the N-terminus of mature salmon/trout IGF-II

State and Appearance:

Lyophilized powder

Specificity:

Suitable for detecting IGF-II in Onchorhynchus and Salmo sp.

As determined by EIA:
5.6 % cross-reactivity with salmon insulin
< 0.1 % cross-reactivity with recombinant salmon/trout IGF-I (Oncorhynchus kisutch & Oncorhynchus mykiss), recombinant salmon/trout growth hormone (Oncorhynchus kisutch & Oncorhynchus mykiss), recombinant tuna IGF-I (Thunnus maccoyii), recombinant tuna IGF-II (Thunnus maccoyii), recombinant bream IGF-I (Pagrus auratus), recombinant bream growth hormone (Acanthopagrus butcheri), recombinant barramundi IGF-I (Lates calcarifer), recombinant human IGF-I, recombinant human IGF-II, recombinant rat IGF-I, recombinant rat IGF-II, recombinant rat growth hormone

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve in a suitable buffer appropriate for the desired application

Application and Titre:

EIA 1/1,000
Immunoblotting 1/1,000

Protocols:

#3020 General Procedure for Microplate Enzyme Immunoassay (EIA)

#4120 Procedure for Enzyme Immunoassay (EIA) of Salmon/Trout IGF-II

Related Products:

Salmon/Trout IGF-II

Salmon / Trout IGF-II Pack

Product Performance:
      reactivity: