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References:

Duguay, S.J. (1999) Horm. Metab. Res. 31, 43-49

Source:

Rabbit antiserum

Immunogen:

Synthetic peptides corresponding to amino acids 78-88, 89-101 or 138-156 of the human proIGF-II precursor protein.

State and Appearance:

Lyophilized powder

Specificity:

As determined by Western Blotting:
The three antisera recognize the IGF-II precursor protein but do not cross-react with mature human IGF-II.

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C or -80°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve in 500 µl sterile water (a 1/5 dilution). Dilute in a buffer suitable for the desired application

Application and Titre:

Western Blotting 1/4,000 IGF-IIE (77-88) and IGF-IIE (89-101)
Western Blotting 1/8,000 IGF-IIE (138-156)
Enzyme Immunoassay 1/5,000

Protocol:

#3009: Procedure for Western Immunoblotting human ProIGF-II

Related Products:

Human proIGF-II (aa 1-156)

IGF-IIE, (aa 138 - 156), anti-human

IGF-IIE, (aa 78 - 88), anti-human