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References:

Liu, S. et al. (1991) Biochem. Biophys. Res. Communs. 174, 673-679
Schuller A.G. et al. (1993) Growth Regulation 3, 32-34

Source:

Rabbit antiserum

Immunogen:

Epitope of human IGFBP-1

State and Appearance:

Lyophilized powder

Specificity:

As determined by EIA:
Antibody does not recognize hIGFBPs 2,3,4,5 or 6

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C or -80°C
Avoid freeze-thaw cycles

Reconstitution:

Dissolve in 250 µl sterile water (a 1/10 dilution). Dilute in a buffer suitable for the desired application

Application and Titre:

Enzyme immunoassay 1/1,000
The antiserum reacts with both the phosphorylated and non-phosphorylated forms of IGFBP-1.
Half-maximal inhibition (ED₅₀) of radioligand binding.
Western Blotting 1/1,000

Protocol:

#3110: Protocol for Western Immunoblotting using human IGFBP antisera

Related Products:

Human IGFBP-1 (Native)

Human IGFBP-1 Pack

IGFBP-1, antibody for Immunohistochemistry

IGFBP-1 Immunohistochemistry Pack