Human Insulin Receptor antibody 83-7 is a murine monoclonal antibody (IgG1) that recognizes an epitope within amino acids 140-301 (the cysteine rich region) of the extracellular domain of the human insulin receptor. The antibody enhances the binding of 125I-insulin binding to the insulin receptor of HIR3.5/3T3 cells and stimulates insulin-mediated 3H-thymidine incorporation in these cells.
References:
Soos, M.A. et al. (1992) Biochem. J. 235, 199-208
Source:
Murine monoclonal antibody
Immunogen:
Transfected 3T3 cells overexpressing the human Insulin Receptor
State and Appearance:
Lyophilized powder
Specificity:
Does not cross-react with the rat Insulin Receptor or the human Type 1 IGF Receptor. Biological Activity:
The antibody enhances insulin binding to the Insulin Receptor of 3T3 cells and insulin-stimulated 3H-thymidine incorporation in these cells.
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
After reconstitution store at -20°C or -80°C
Avoid freeze-thaw cycles
Reconstitution:
Dissolve in 200 µl phosphate buffered saline pH 7.4
