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Description:
Bovine Betacellulin (BTC), a member of the EGF family, is an 80 amino acid heparin-binding protein. Like other members of the EGF family, it is synthesized as a transmembrane precursor. The soluble cytokine, containing one EGF structural motif, is released by proteolytic cleavage. BTC can bind to the EGF receptor (ErbB-1) which then dimerizes with another member of the ErbB receptor family to signal through the tyrosine kinase pathway. BTC is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells and is the only member of the EGF family to stimulate insulin secretion in pancreatic b cells.
References:
Dunbar, A.J. & Goddard, C. (2000) Int. J. Biochem. & Cell Biol. 32, 805-815.
Pinkas-Kramarski R. et al. (1998) Oncogene, 16, 1249-1258
Source:
Produced recombinantly in E. coli
Purity:
By N-terminal sequencing: > 95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC
Molecular Weight:
Theoretical: 8995 daltons
By Mass Spectrometry the product is 50 % [Gly1]BTC (9052 daltons) and 50 % Des(1-4)BTC (8709 daltons)
N-terminal sequence analysis:
5 residues > 95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC
Biological Activity:
Cell growth bioassay in Balb/c 3T3 fibroblasts: ED50< 10 ng/ml
Endotoxin:
< 0.1 EU/µg
State and Appearance:
Lyophilized white powder
Dried from 0.05 M acetic acid, 0.05% (v/v) trifluoroacetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa)
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
Reconstitution:
#1500: Handling of Novozymes GroPep EGF, TGF-α and Betacellulin (BTC)
Detection:
#3500: Procedure for Iodination of EGF / Betacellulin Peptides
#3501: Determination of EGF / Betacellulin by Radioimmunoassay (RIA)
#3520: Procedure for Western Immunoblotting of EGF / Betacellulin
Related Products:
Betacellulin, anti-bovine
Bovine Betacellulin pre-RIA Pack
Bovine Betacellulin Pack
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