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Description:

Human Transforming Growth Factor-Alpha (TGF-α) is a non-glycosylated, single chain, 50 amino acid polypeptide which is a member of the Epidermal Growth Factor (EGF) family of proteins. It promotes the proliferation and differentiation of cell types displaying the EGF receptor. The biological effects of human TGF-α are likely to be mediated by autocrine and paracrine mechanisms in adult and embryonic cells as well as in a variety of tumors and retrovirally transformed cells.

References:

Dunbar, A.J. & Goddard, C. (2000) Int. J. Biochem. & Cell Biol. 32, 805-815.
Groenen L. C. et al. (1994) Growth Factors, 11, 235-257

Source:

Produced recombinantly in E.coli

Purity:

> 95 % (by HPLC and N-terminal sequence analysis)

Molecular Weight:

5546 daltons – confirmed by Mass Spectrometry

N-terminal sequence analysis:

5 residues > 95 % single sequence

Biological Activity:

Cell growth bioassay in Balb/c 3T3 fibroblasts: ED₅₀ 2.5 ng/ml
EGF radioreceptor assay in AG2804 fibroblasts: ED₅₀ 10 ng/ml

Endotoxin:

< 0.1 EU/µg

State and Appearance:

Lyophilized white powder
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum

Storage/Stability:

At least 2 years at 2 - 4°C (lyophilized)

Reconstitution:

#1500: Handling of Novozymes GroPep EGF, TGF-α and Betacellulin (BTC)

Detection:

#3530 Procedure for Enzyme Immunoassay (EIA) of TGF-α

Related Products:

TGF-á, anti-human

Human TGF-á Pack

Human LONG™TGF-á

Product Performance:
      Radioreceptor Assay:




      Type II Radioreceptor Assay:




      Radioreceptor Assay for EGF: