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Description:
Red Sea bream (Pagrus auratus) Insulin-like Growth Factor-I (rbrIGF-I) is a 67 amino acid polypeptide. Recombinant red sea bream (Pagrus auratus) IGF is identical to [Gly1] gilthead sea bream (Sparus aurata) IGF-I and [Gly1] black bream (Acanthopagrus butcheri) IGF-I, as glycine replaces the serine at position 1 in these IGFs.
References:
Duguay S.J. et al. (1996) J. Mol. Endocrinol., 16, 123-132
Source:
Produced recombinantly in E.coli
Purity:
>90 % (by HPLC and N-terminal sequence analysis)
Molecular Weight:
7379 daltons – confirmed by Mass Spectrometry
N-terminal sequence analysis:
5 residues > 95 % single sequence
Biological Activity:
Stimulation of protein synthesis in rat L6 myoblasts:
ED50 <50 ng/ml
Endotoxin:
< 0.1 EU/µg
State and Appearance:
Lyophilized white powder
Dried from 0.1 M acetic acid and stored under nitrogen at a slight vacuum
Storage/Stability:
At least 2 years at 2 - 4°C (lyophilized)
Detection:
By RIA
Reconstitution:
#1000: Handling of GroPep Bioreagents IGF-I, IGF-II and IGF Analogs
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