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Description:

Suitable for ELISA

Contains:
Human LONG®R³IGF-I ELISA Standard, Product Code: BK01
1 x vial Human LONG®R³IGF-I Double monoclonal ELISA Coating Antibody, Product Code: MAN1
1 x vial Human LONG®R³IGF-I Double monoclonal ELISA Detection Antibody, Product Code: MAO1 (Biotin-labelled)

One Pack contains sufficient reagents for 1 x 96 well ELISA plate

Immunogen:

Human LONG®R³IGF-I

Specificity:

The assay detects Human LONG®R³IGF-I and is calibrated against the Media Grade of this reagent
Cross-reactivity with human IGF-I is 32 %
No significant cross-reactivity to human IGF-II

No interference was observed from culture media containing up to 10 % fetal bovine serum

Reconstitution:

Human LONG®R³IGF-I ELISA Standard: 1 ml of 10 mM HCl
Human LONG®R³IGF-I Double monoclonal ELISA Coating Antibody: 1 ml of PBS, pH 7.4
Human LONG®R³IGF-I Double monoclonal ELISA Detection Antibody: 1 ml of PBS, pH 7.4

Protocol:

#3030 Procedure for Human LONG®R³IGF-I Double monoclonal ELISA (BK / MAN1 / MAO1)